A green strategy to produce potential substitute resource for bear bile using engineered Saccharomyces cerevisiae

Abstract Background Bear bile powder is a precious natural material characterized by high content of tauroursodeoxycholic acid (TUDCA) at ratio 1.00–1.50 to taurochenodeoxycholic (TCDCA). Results In this study, we use the crude enzymes from engineered Saccharomyces cerevisiae directionally convert TCDCA chicken TUDCA committed in vitro. This S. strain was modified with heterologous 7α-hydroxysteroid dehydrogenase (7α-HSDH) and 7β-hydroxysteroid (7β-HSDH) genes. host HSDH gene combinatorial optimization response surface methodology applied get best optimal biotransformation condition, respectively, under which 10.99 ± 0.16 g/L products containing 36.73 6.68% 28.22 6.05% were obtained using 12.00 as substrate. Conclusion study provides healthy environmentally friendly way produce potential alternative resource for bear cheap readily available powder, also gives reference green manufacturing other rare endangered animal-derived valuable resource.